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KMID : 0894520060100020097
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Development & Reproduction
2006 Volume.10 No. 2 p.97 ~ p.103
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Effect of Growth Hormone and Androgen on Vitellogenin and Estrogen Receptor Gene Expression in the Japanese eel, Anguilla japonica
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Kwon Hyuk-Chu
Choi Seong-Hee
Kim Eun-Hee
Kwon Joon-Yeong
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Abstract
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Vitellogenin(Vg) is a sex specific serum protein present in sexually maturing female blood of oviparous vertebrates. Estrogen(E₂) is a main inducer of hepatic Vg synthesis. We investigated the effects of androgen and growth hormone(GH) on regulation of Vg and estrogen receptor(ER) genes in Japanese eel. Immature eels(200～250 g) were given a single injection of E₂(5～5,000㎍/㎏ㆍbw) alone, or in combination with eel recombinant GH(eGH, 1～10㎍/㎏) or methyltestosterone(MT, 1～5 ㎎/㎏) and sacrificed 10 days after the hormone treatments. Expression levels of ER and Vg genes from the liver were determined by means of reverse transcription and polymerase chain reaction(RT-PCR). Administration of E₂ stimulated Vg gene expression in a dose dependent manner. Levels of Vg mRNA after the injection of E2(500㎍/㎏) with MT(5㎎/㎏) or eGH(10㎍/㎏) were much higher than in that of E2 alone(500㎍/㎏). Whereas, injection of either vehicle, eGH (10㎍/㎏) or MT(5㎎/kg) alone did not induce the expression of Vg gene in the liver. ER mRNA was detected from the fish treated with vehicle alone. E2 injection(5～500㎍/㎏ㆍbw) increased this ER expression but dose dependent response was not clear. Addition of MT(5㎎/㎏) or eGH(10㎍/㎏) did not affect E₂- stimulated ER mRNA expression. This study confirms the necessity of E₂ as the primary factor for Vg gene expression and requirement of additional hormones such as MT or GH for the full expression of Vg mRNA, and suggests that the additive effect of MT or GH on Vg gene expression would be mediated by some unknown factors other than ER.
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KEYWORD
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Eeel, Vitellogenin, Estrogen receptor, Growth hormone, Androgen, RT-PCR
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